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Identification of the characteristics <t>of</t> <t>sEVs.</t> (a‐c) Transmission electron microscopy (TEM), nanoparticle tracking analysis (NTA) and western blotting were used to examine the characteristics of sEVs continuously collected for 5 days. (d‐f) TEM, dynamic light scattering <t>(DLS)</t> and CytoFLEX flow cytometer were used to examine the characteristics of sEVs extracted on day 1, day 3, and day 5. (a) TEM results of sEVs. M‐UC‐ex represents sEVs extracted by M‐UC; EQ‐ex represents the sEVs extracted by EQ. Bar = 200 nm. (b) NTA results of sEVs derived from hiPSCs‐EC1. (c) The western blotting results. HSP70, CD63, and TSG101 are the exosome marker proteins; Calreticulin is the negative control. (d) TEM results of sEVs. Bar = 200 nm. (e) DLS results of sEVs. (f) CytoFLEX flow cytometer results of sEVs derived from hiPSCs‐EC1
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Identification of the characteristics of sEVs. (a‐c) Transmission electron microscopy (TEM), nanoparticle tracking analysis (NTA) and western blotting were used to examine the characteristics of sEVs continuously collected for 5 days. (d‐f) TEM, dynamic light scattering (DLS) and CytoFLEX flow cytometer were used to examine the characteristics of sEVs extracted on day 1, day 3, and day 5. (a) TEM results of sEVs. M‐UC‐ex represents sEVs extracted by M‐UC; EQ‐ex represents the sEVs extracted by EQ. Bar = 200 nm. (b) NTA results of sEVs derived from hiPSCs‐EC1. (c) The western blotting results. HSP70, CD63, and TSG101 are the exosome marker proteins; Calreticulin is the negative control. (d) TEM results of sEVs. Bar = 200 nm. (e) DLS results of sEVs. (f) CytoFLEX flow cytometer results of sEVs derived from hiPSCs‐EC1

Journal: Journal of Extracellular Vesicles

Article Title: Optimized culture methods for isolating small extracellular vesicles derived from human induced pluripotent stem cells

doi: 10.1002/jev2.12065

Figure Lengend Snippet: Identification of the characteristics of sEVs. (a‐c) Transmission electron microscopy (TEM), nanoparticle tracking analysis (NTA) and western blotting were used to examine the characteristics of sEVs continuously collected for 5 days. (d‐f) TEM, dynamic light scattering (DLS) and CytoFLEX flow cytometer were used to examine the characteristics of sEVs extracted on day 1, day 3, and day 5. (a) TEM results of sEVs. M‐UC‐ex represents sEVs extracted by M‐UC; EQ‐ex represents the sEVs extracted by EQ. Bar = 200 nm. (b) NTA results of sEVs derived from hiPSCs‐EC1. (c) The western blotting results. HSP70, CD63, and TSG101 are the exosome marker proteins; Calreticulin is the negative control. (d) TEM results of sEVs. Bar = 200 nm. (e) DLS results of sEVs. (f) CytoFLEX flow cytometer results of sEVs derived from hiPSCs‐EC1

Article Snippet: The average particle dimensions and concentration of the sEVs were characterized by the DLS technique (ZetaView, Particle Metrix).

Techniques: Transmission Assay, Electron Microscopy, Western Blot, Flow Cytometry, Derivative Assay, Marker, Negative Control